ABSTRACT
Objective To investigate the molecular mechanism of osteoclast differentiation induced by staphylococcal lipoteichoic acid (LTA-sa). Methods Raw264.7 cells were treated with LTA-sa in a concentration of 200 ng/mL for 0, 5, 10, 20, 40, 60 min and 0, 1, 2, 3 days respectively, and the proteins in signaling pathways associated with osteoclast differentiation were measured with western blot. In addition, Raw264.7 cells were treated with different concentrations of LTA-sa (100, 200 and 400 ng/mL) and PBS for 0, 1, 2, 3 days, the expression of TNF-α, IL-1α and IL-6 was detected with Enzyme linked immunosorbent assay (ELISA). Results (1)Western blot showed that, under stimulation of LTA-sa, IκB-α decreased at 5 min and 10 min, while the phosphorylation of nuclear factor κB increased at 10 min . In addition , NFATc1 increased in 2 and 3 days gradually. The above results were statistically analyzed, and the difference was significant in statistics (P < 0.001). (2)ELISA showed that the expression of IL-6 increased in 2 and 3 days along with the increasing concentration and prolonging stimulation time of LTA-sa. Data were statistically analyzed, the difference was significant in statistics (P < 0.001). Conclusion LTA-sa promotes osteoclast differentiation through the NF-κB signaling pathway and the secretion of IL-6.
ABSTRACT
Objective To investigate the clinical efficacy of complete mesocolic excision (CME)in patients with colonic cancer,so as to provide a theoretical basis for further development of CME.Methods A total of 92 patients with colonic cancer were collected.The patients were divided into CME group and control group according to the operation method with 46 patients each.The operating time,intraoperative blood loss,after bed time,passage of flatus time,the first feeding time,postoperative hospital stay,numbers of lymph node dissection and postoperative complications were compared between two groups.Results There were no significant differences in the operating time,after bed time,passage of flatus time,the first feeding time and postoperative hospital stay between two groups(P＞ 0.05).However,there was significant difference in intraoperative blood loss and numbers of lymph node dissection between CME group and control group[(269.7 ±32.5)ml vs.(367.8 ±41.2)ml,(19.7 ±4.2)numbers vs.(13.6 ±3.4)numbers](P＜0.05).There was no significant difference in the postoperative complications between two groups(P ＞ 0.05).Conclusions CME shows more clinical efficacy in colonic cancer,and which does not affect postoperative recovery and postoperative complications.CME is worthy to promote the clinical application in colonic cancer.